P10 - Basic Fibroblast Growth Factor (B-FGF) Expression and Flap Viability Following Surgical Delay of a Rat Transverse Rectus Abdominis Myocutaneous (Tram) Flap

Room 2 (Henry B. Gonzalez Convention Center)
Sunday, November 3, 2002
8:00 AM - 4:00 PM
Room 2 (Henry B. Gonzalez Convention Center)
Monday, November 4, 2002
8:00 AM - 4:00 PM
Room 2 (Henry B. Gonzalez Convention Center)
Tuesday, November 5, 2002
8:00 AM - 4:00 PM
Room 2 (Henry B. Gonzalez Convention Center)
Wednesday, November 6, 2002
8:00 AM - 4:00 PM

311

P10 - Basic Fibroblast Growth Factor (B-FGF) Expression and Flap Viability Following Surgical Delay of a Rat Transverse Rectus Abdominis Myocutaneous (Tram) Flap

Michael S. Wong, MD, Detlev Erdmann, MD, Ranya Sweis, Christiane Pollmann, Gregory S. Georgiade, MD, L. Scott Levin, MD, Kevin C. Olbrich, PhD, and Bruce Klitzman, PhD.

Background: Partial TRAM flap loss in breast reconstruction can be a devastating complication. Surgical delay of the TRAM flaps has been shown to improve its viability. b-FGF is a well-known, potent angiogenic molecule.
Objective: To study the expression of b-FGF and flap viability in surgically delayed rat TRAM flaps.
Methods: 35 female Sprague-Dawley rats were randomized to 1 of 4 groups: no delay (N=6), 7-day delay (N=12), 14-day delay (N=10), and 21-day delay (N=7). Surgical delay consisted of incising the perimeter skin of the planned TRAM flap followed by ablation of both superior epigastric arteries and the left inferior epigastric artery, thus preserving the right inferior epigastric artery, and raising all but Zone I off the abdominal wall fascia. Three days following TRAM procedure, all rats were euthanized. Post-fluoroscein planometry determined percent area viability of both superficial and deep portions of TRAM flaps. Additionally, full thickness TRAM specimens were taken from Zones I- IV for b-FGF ELISA analysis. Analysis of variance and the student T-test were used.
Results: b-FGF levels (pg b-FGF/mg protein) are presented as the mean+SEM

Delay

Zones

(Days)

I

II

III

IV

0

41.3+16.2

61.3+19.4

27.2+11.3

24.1+15.0

7

93.2+15.1

88.2+15.2

86.1+14.5

94.0+15.3

14

93.8+10.5

94.5+10.4

75.5+5.9

77.5+11.1

21

75.9+11.2

66.0+8.2*

77.3+8.7

79.2+11.3

All delayed flaps (*except Zone II, 21-day delay) had significantly higher b-FGF levels compared to non-delayed flaps (p < 0.05). Among delayed flaps, b-FGF levels did not differ with length of delay nor flap zone. All delayed flaps had a significantly higher area of flap viability superficially than non-delayed flaps (p < 0.05). There was no difference in deep flap viability.

Conclusion: Surgical delay of TRAM flaps is associated with improved flap viability and significantly elevated levels of b-FGF over non-delayed TRAMS. Increases in b-FGF may contribute to improved TRAM flap viability following delay surgery.
View Synopsis (.doc format, 330.0 kb)

Background: Partial TRAM flap loss in breast reconstruction can be a devastating complication. Surgical delay of the TRAM flaps has been shown to improve its viability. b-FGF is a well-known, potent angiogenic molecule. Objective: To study the expression of b-FGF and flap viability in surgically delayed rat TRAM flaps. Methods: 35 female Sprague-Dawley rats were randomized to 1 of 4 groups: no delay (N=6), 7-day delay (N=12), 14-day delay (N=10), and 21-day delay (N=7). Surgical delay consisted of incising the perimeter skin of the planned TRAM flap followed by ablation of both superior epigastric arteries and the left inferior epigastric artery, thus preserving the right inferior epigastric artery, and raising all but Zone I off the abdominal wall fascia. Three days following TRAM procedure, all rats were euthanized. Post-fluoroscein planometry determined percent area viability of both superficial and deep portions of TRAM flaps. Additionally, full thickness TRAM specimens were taken from Zones I- IV for b-FGF ELISA analysis. Analysis of variance and the student T-test were used. Results: b-FGF levels (pg b-FGF/mg protein) are presented as the mean+SEM
Delay	Zones
(Days)	I	II	III	IV
0	41.3+16.2	61.3+19.4	27.2+11.3	24.1+15.0
7	93.2+15.1	88.2+15.2	86.1+14.5	94.0+15.3
14	93.8+10.5	94.5+10.4	75.5+5.9	77.5+11.1
21	75.9+11.2	66.0+8.2*	77.3+8.7	79.2+11.3