Introduction: Prolonged ischemia leads to alterations in the tecidual microcirculation and oxigen free radicals production. This event is called no reflow phenomenon and its management is a challenge to the microsurgeon dealing with replantation. It was tested a new replantation model after warm ischemia in rats and the effectiveness of allopurinol, streptoquinase and hyperbaric oxigen therapy. Method: Amputation with preservation of vessels and nerves of the right hind limb was proceeded in 110 rats. The groups MG1, MG2, MG3, MG4 and MG5 were submitted to 0, 2, 4, 6 and 8 hours of ischemia. The limbs were observed for 7 days and the survival limb rates in each group were 100%, 80%, 63,6%, 50% and 20% respectively. MG4 was elected control for experimental groups. The groups EG1, EG2 and EG3 were submitted to 6 hours of ischemia and were treated with streptoquinase , allopurinol and hyperbaric oxigen therapy. The survival limb rates were 66,7%, 70% and 30% respectively. The results were tested using Q-square test and p<0,05 was considered statistically significant. Results: MG1, MG2, MG3, MG4 and MG5 were statistically different except for MG3 and MG4. EG1 had a better limb survival than MG4 but not than MG3. EG2 had a better limb survival than MG4 but not than MG2 and MG3. EG3 had a worse limb survival than MG4. Discussion: The results suggest that the administration of streptoquinase and allopurinol may be helpful to improve limb salvage after ischemia and hyperbaric oxigen therapy may not be helpful and even can be harmful to ischemic limbs.

View Synopsis (.doc format, 983.0 kb)