Introduction: The purpose of this study is to evaluate changes in the skin following microdermabrasion using a porcine model. Methods: An 8-week-old pig, four areas were tattooed as A, B, C, and D. These areas were subdivided, in a grid like fashion, into 3x3cm squares and numbered 1-8. Control squares were chosen, one from each area. We initiated the vacuum of the machine at 25 mmHg the first week, and increasing the vacuum by 5 mmHg for each of 7 weeks up to 55 mmHg, over a seven-week period. Biopsies of each grid were obtained weekly. Biopsies were stained with H&E, and Verhoff -Van Geison (Elastin Stain). Histometric analyses of the skin biopsies were performed using a digital imaging system. Results: The ratio of dermal, collagen bundle, and epidermal thickness were increased in the treated groups. The dermal thickness was increased in the thinner skin by 40%, and 29% in the thicker skin. Similarly, the increase in the collagen bundle thickness was 22%, while increase in the epidermal thickness was 9%. Conclusion: Microdermabrasion produces an increase in dermal thickness, collagen bundle thickness, and epidermal thickness. We believe that this methodology is an effective tool to quantify those changes.