Purpose: Present the clinical experience of 22 patients treated with injections of cultured fibroblasts for facial rejuvenation by correcting small soft tissue defects in the face, acne scarring,nasal labial folds and facial wrinkles.

Material and Methods: Twenty two patients were treated from February 2002 to the present time. A small elliptical skin excision of 3 mm is removed from the retro auricular area. This biopsy is sent to the manufacturer's laboratory were fibroblasts are extracted and grown over a six week period. A 1 cc vial of ready made patient's fibroblasts is sent back to the doctor's office to be injected to specific areas in the face.

A sequence of 3 injections are performed by infiltrating into the dermis through a 30 gauge needle. Twenty five per cent of patients received a placebo and the remaining 75% received cultured fibroblasts at different concentrations:

1.- 25% of patients received a placebo injection. 2.- 25% of patients received an injection with a concentration of 5 million/ml. 3.- 25% of patients received an injection with a concentration of 10 million/ml 4.- 25% of patients received an injection with a concentration of 20 million/ml.

Results: Results were measured by the patients and the doctor on a grading scale and by a sliding scale. The dose of 20 million/ml produced the most significant and satisfying results.

Conclusions: We are optimistic with these findings and feel that further research is mandatory in order to recommend this customized approach to treat not the signs and symptoms of the condition but to correct the underlying disease (lack or reduced amount of fibrin and collagen)