Introduction Previous studies on pulsed magnetic fields (PMF) have reported enhanced fracture and chronic wound healing, endothelial cell growth, and angiogenesis, among other applications. This study characterized the biomechanical changes that occur when standard cutaneous wounds are exposed to radio frequency PMF with specific dosage parameters, in an attempt to determine whether return to functional tensile strength could be accelerated in the wound-healing process. Methods The study was carried out in two phases on a total of 100 rats. In phase I, wounds were exposed to a high amplitude (1.0 G) PMF signal in clinical use for wound repair for 21 or 60 days. Forty Sprague-Dawley male rats were divided into four groups of 10 animals each, two treatment groups and two sham treatment groups. Each active/sham pair was assigned to a time point. Phase II was designed as a prospective, placebo-controlled double-blinded trial in which 60 rats were divided into three sham-treated groups and three active groups of 10 each treated with three different low amplitude (0.02-0.05 G) PMF signals, configured assuming a Ca2+ binding transduction pathway for 21 days. A midline 8-cm linear skin incision was performed on the dorsum of each rat in the study. Tensile strength was determined by measuring the point of rupture of the wound on a standard tensiometer loaded at 0.45 mm/sec. Results The mean tensile strength of treated groups in phase I was 48% (p<0.001) greater than controls at 21 days, while there was no significant difference at 60 days. In phase II, the treated groups showed 18% (NS), 44%, and 59% (p<.001) increases of tensile strength over controls at 21 days. Conclusions The authors successfully demonstrated that exposing wounds to pulsed magnetic fields of very specific configurations accelerated early wound healing in this animal model, as evidenced by significantly increased wound tensile strength at 21 days post wounding.