Background:-Keloid is an exuberant inflammatory response to skin injury due to abnormal Keratinocytes and Fibroblast interaction and their differentiation, appearing as disfiguring skin lesion extending beyond the actual injuring stimuli. Beta Defensin Proteins are primarily a group of innate immunoglobulins against microbial agents but are also involved in cell differentiation, cell signalling and triggering the adoptive immune system. Aim: We hypothesise that Beta Defensin Proteins are involved in keloid pathogenesis as keloid has microenvironment of dense cytokine secretions and chronic inflammatory response. To my knowledge no previous study has examined the relationship of Beta Defensin and Keloid formations. Methods: The keloid and normal skin samples were collected from patients undergoing plastic surgery procedures. The samples were snapped frozen and subjected to immunohistochemistry with antibodies against Defensin Beta1, 2 and 3. Results:Immunohistochemistry analysis of ten Keloid and site specific normal skin showed that Beta Defensin 1 and 3 are has increased expression in keloid as compare to site specific skin. Conclusion.Dense secretion of Defensin Beta 1 and 3 at Stratum Corneum lead to the abnormal differentiation of keloid keratinocytes which hyperproliferate in epidermis and influence keloid fibroblast to hyper secrete the cytokines in chronic inflammatory milieu.