Comparison of Bone Regeneration In Rabbit Bone Marrow Stem Cells and Adipose Stem Cells

Comparison of bone regeneration in rabbit bone marrow stem cells and adipose stem cells

Purpose: The occurrence of stem cell differentiation in every mesenchymal cell is a current topic of research interest. This study analyzed stem cell-derived optimization of differentiation and efficient regeneration by harvesting bone marrow- and adipose-derived stem cells. In addition, bone regeneration in bone marrow and adipose stem cells is researched and compared.

Methods and meterials: Stem cells are harvested from 20 white NewZealand rabbits raised in the same environment. The experiment involved craniectomy to produce 10X10㎜sized calvarial bone defects in the rabbits after induction of bone regeneration and injection of 1X10⁶cells/L of marrow and adipose stem cells, respectively(Fig. 1). After 6 week, bone regeneration was noted by visible observation, volume changes of harvesting bones, 3-dimensional skull bone computed tomography and histological analysis.

Results: Both bone marrow stem cells and adipose stem cells are effective for bone regeneration. The amount of regenerated bone was slightly greater in bone marrow stem cells than adipose stem cells, but the difference was not significant(Table 1).

Conclusions: Compared with bone marrow stem cells, adipose stem cells are more effective bone regenerating material because of their easy accessibility, multi-differentiated ability, and capability to form multiplicated stem cells through several cycles of subculture.

Disclosure/Financial Support

The author has no financial interest to declare in relation to the content of this article.

Fig. 1. Intraoperative photography of rabbit skull bone with two craniectomies. The one put into bone marrow stem cell+fibrin glue, the other was adipose-derived stem cell+fibrin glue respectively.