Abstract: The Effect of Combined Use of Platelet Rich Plasma and Adipose Derived Stem Cells On Fat Graft Survival (Plastic Surgery 2012)

Sunday, October 28, 2012: 9:00 AM

Nevra Seyhan, MD , Department of Plastic Surgery, Konya University, Konya, Turkey

Dogan Alhan, MD , Department of Plastic Surgery, Gulhane Military Medical Academy, Ankara, Turkey

Ali Ugur Ural, MD , Department of Hematology, Gulhane Military Medical academy, Turkey

Armagan Unal, MD , Department of Pathology, Gulhane Military Medical Academy, Turkey

Mustafa Cihad Avunduk, MD , Department of Pathology, Konya University, Konya

Nedim Savaci, MD , Department of Plastic Surgery, Konya University, Konya, Turkey

Introduction: The efficacy of autologous fat transplantation is reduced by fat absorbsion and fibrosis due to fat necrosis.The effect of platelet rich plasma, adipose derived stem cell and their combination on fat graft survival was investigated in this study.

Methods: In the experiment, inbred Ficher 344 rats were used for isogenous form of transplantation. Rats were seperated into 4 groups (n=10). Platelet rich plasma(PRP) was prepared from blood obtained from 4 donor rats. Inguinal fat pads were harvested. Adipose derived stem cells were isolated,expanded three passages and labeled with fluorescent DýI. Fat tissues were mixed with 0.2 ml Dulbecco’s modified Eagle Medium in group A, 0.2 ml PRP in Group B, 0.2 ml of 5x10⁶DiI labeled adipose derived stem cell(ADSC) in group C,and a combination of PRP and ADSC in group D. All four types of fat preparations were injected subcutaneously into the scalp of the rats. After 3 months the fat grafts were extirpated and weighed. The volumes of the transplants were measured by the liquid overflow method

Tissue sections from the center of the dissected fat biopsy were stained with hematoxylin-eosin, triple and VEGF. The histologyc parameters evaluated included the number of capillary structures, cyst vacuoles, fibroblasts and intact adipocytes.The number of each parameter were counted on an area of 335076.3µm² by Clemex Vision lite 3,5 visual analysing programme. In vitro growth factor (VEGF, TGFβ,FGF) levels of supernatant were compared between stem cell and stem cell plus platelet rich plasma group using the enzyme- linked immunosorbent assay method

Results: In group D there was more grafted fat retained 3 months later. The loss of graft mass was more significant in group A than the other groups. The number of capillary structures and intact adipocytes were significantly higher in group D. Less cyst formation and fibrosis was obtained in group D . Group C and D transplants exhibited the histological structure of normal fat tissue.

The level of growth factors were significantly higer in stem cell plus platelet rich plasma group.

Conclusion: the combined use of adipose derived stem cells and platelet rich plasma synergistically increase revascularization and fat graft survival.

21276 The Effect of Combined Use of Platelet Rich Plasma and Adipose Derived Stem Cells On Fat Graft Survival