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Saturday, October 12, 2013: 1:25 PM
Background. Supplementation of fat grafts with stromal vascular fraction (SVF) cells is an emerging technique used to improve graft reliability. A variety of systems for isolating SVF are commercially available. The lack of performance data obtained operating the systems in a standardized environment prevents objective assessment of performance. This prospective, blinded study compared performance of four commercially available SVF isolation systems when operated in a clinical outpatient surgery environment.
Methods. Four different systems were compared: 1) PNC’s Multi-Station, 2) CHA Biotech Cha Station™, 3) Cytori Celution®800/CRS System, and 4) Medi-Khan’s Lipokit™ with MaxStem (Lipokit). Identical lipoaspirate samples from 5 separate volunteer donors were used to evaluate system process time, viable cell yield, composition, residual enzyme and operating costs. Results. The mean processing time ranged from 88 to 115 minutes. The highest mean number of viable nucleated cells was obtained using the Celution System (2.41x105 cells/gram) followed by the Multi-Station (1.07x105 cells/gram). Lipokit and Cha Station systems yielded nearly a log fewer nucleated cells (0.35x105 cells/gram and 0.05x105 cells/gram, respectively). The Celution System also yielded a significantly greater yield of endothelial cells, CD34+/CD31-cells, and adipose derived stem cells (CFU-F). Residual enzyme levels observed with the Multi-Station, Cha Station, and Lipokit respectively averaged 5.1, 13.0, and 57-fold higher than that observed with the Celution system.
Methods. Four different systems were compared: 1) PNC’s Multi-Station, 2) CHA Biotech Cha Station™, 3) Cytori Celution®800/CRS System, and 4) Medi-Khan’s Lipokit™ with MaxStem (Lipokit). Identical lipoaspirate samples from 5 separate volunteer donors were used to evaluate system process time, viable cell yield, composition, residual enzyme and operating costs. Results. The mean processing time ranged from 88 to 115 minutes. The highest mean number of viable nucleated cells was obtained using the Celution System (2.41x105 cells/gram) followed by the Multi-Station (1.07x105 cells/gram). Lipokit and Cha Station systems yielded nearly a log fewer nucleated cells (0.35x105 cells/gram and 0.05x105 cells/gram, respectively). The Celution System also yielded a significantly greater yield of endothelial cells, CD34+/CD31-cells, and adipose derived stem cells (CFU-F). Residual enzyme levels observed with the Multi-Station, Cha Station, and Lipokit respectively averaged 5.1, 13.0, and 57-fold higher than that observed with the Celution system.
Conclusions. While all systems generated measurable amounts of SVF, significant variability exists in the number, identity, and safety profiles of recovered viable cells. Side-by-side clinical trials will be required to establish the relevance of these differences.