Abstract: Adipose-Derived Aldehyde-Dehydrogenase-Expressing Cells Accelerate Re-Vascularization of Collagen-Glycosaminoglycan Scaffolds (Plastic Surgery The Meeting)

Saturday, September 24, 2016

Hajime Matsumine, MD, PhD , Department of Plastic and Reconstructive Surgery, Tokyo Womens Medical University, Tokyo, Japan

Kazuyuki Numakura, MD, PhD , Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA

Satoshi Tsunoda, PhD , Division of Interdisciplinary Medicine and Biotechnology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA

Huan Wang, MD , Department of Surgery, Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA

Rui Matsumine, MD, PhD , Division of Interdisciplinary Medicine and Biotechnology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA

Mihail Climov, MD , Department of Surgery, Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA

Giorgio Giatsidis, MD , Department of Surgery, Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA

Vikas P Sukhatme, MD, ScD , Division of Interdisciplinary Medicine and Biotechnology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA

Dennis P Orgill, MD, PhD , Department of Surgery, Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA

Presentation PDF file

E-Poster

Introduction: Collagen-glycosaminoglycan (CG) scaffolds, also known as dermal regeneration templates, are used for the reconstruction of full-thickness skin defect in patients with extensive resections due to burns, traumatic or inflammatory conditions. The natural process of revascularization typically lasts two to three weeks precluding immediate skin grafting. The usage of stem cells has demonstrated to accelerate wound healing. However, stem cell usage requires several steps of cell culturing that preclude using this method in the acute setting. Aldehyde dehydrogenase (ALDH) is an enzyme that plays an important role in retinoid metabolism and is highly expressed in stem cells. In this study, we isolated ALDH-expressing cells from subcutaneous adipose tissue and tested them for their potential to enhance healing in a full-thickness skin wound in rats by co-implanting them with CG scaffolds.

Materials & Methods: Stromal-Vascular-Fraction (SVF) was obtained from subcutaneous adipose tissue of syngeneic rats. ALDH^hi cells were isolated using a fluorescence-activated cell sorting technique with ALDEFLUOR assay kit^TM. Each recipient rat underwent four full-thickness wounds creation on the recipient rat’s back, each wound was treated differently. A total of four treatment groups were formed (n=11). Group 1 (control group) consisted of wounds treated with CG and 100 μL normal saline. Group 2 (SVF group) consisted of CG and 1 × 10⁵cells/cm² SVF cells. Group 3 ( ALDH group) consisted of CG and 1 × 10⁵cells/cm² ALDH^hi cells. Group 4 (ASCs group) consisted of CG and 1 × 10⁵cells/cm² ASCs. Animals were evaluated by histology on day 7 after surgeries.

Results: Scaffolds seeded with ALDH^hi cells histologically demonstrated remarkable enhancements in dermal regeneration, vascularization, and collagen growth, if compared to the wound treated with CG alone, CG with SVF, and CG with ASCs groups. Immunofluorescent staining with CD31 emphasized that transplanted ALDH^hicells differentiated into vascular endothelial cells.

Conclusions: Composite transplantation of CG scaffolds and adipose-derived ALDH^hi cells promoted dermal regeneration, not worse than cultured ASC, suggesting that ALDH^hi cells could be used in an acute setting as a reliable alternative for cultured ASCs.

29315 Adipose-Derived Aldehyde-Dehydrogenase-Expressing Cells Accelerate Re-Vascularization of Collagen-Glycosaminoglycan Scaffolds