37044 Improvement of Tracheal Substitute By Tissue-Engineered Tracheal Adventitia: A New Concept for Tracheal Tissue Engineering

Saturday, September 29, 2018: 9:00 AM
Li Chen, MD , Chang Gung Memorial Hospital Linkou Branch, Taoyuan, Taiwan
Cherng-Shyang Chang, PhD , Chang Gung Memorial Hospital Linkou Branch, Taoyuan, Taiwan
Yi-Shiuan Lin, MS , Chang Gung Memorial Hospital Linkou Branch, Taoyuan, Taiwan
Chung-Kan Tsao, MD , Chang Gung Memorial Hospital Linkou Branch, Taoyuan, Taiwan

INTRODUCTION: Trachea resected patients usually suffer from poor prognosis due to difficulties of reconstruction. Allotransplantation for tracheal replacement is limited by donor shortage, rejection and infection. Tissue engineering could bypass the limitations above by utilizing autologous cells. Previously, we established an ectopic tissue-engineered vascularized trachea based on a poly(ε-caprolactone) (PCL) scaffold with muscle flap surrounding1. The muscle flap protected neo-trachea from degradation; however, the bulky flap may cause dysphagia or limit trachea motion. Thus, we developed a neo-trachea with tissue-engineered tracheal adventitia (TETA) by replacing muscle flap with fibroblast-based adventitia, and tested whether the TETA prevents tracheal collapse and advance the chondrogenesis of the construct.

 

METHODS: The porous PCL scaffold with cylindrical structure was generated by a negative mold. The chondrocytes were seeded onto PCL scaffolds and in vitro-cultured in chondrogenic defined medium for four weeks. The construct was surrounded by fibroblast-based tissue-engineered tracheal adventitia, and was cultivated for 1 week. The PCL neo-trachea was implanted subcutaneously in nude mice for four weeks, and the chondrogenesis of construct were evaluated by histological analysis.

 

RESULTS: The cylindrical structure of TETA-surrounded PCL neo-trachea remained after four-week in vivo cultivation. The immunofluorescence staining revealed that the TETA layer stayed intact without invasion from peripheral tissue to the adventitia or the neo-trachea. In comparison to the neo-trachea without adventitia, the chondrocytes density increased in the construct with TETA surrounding. The cartilaginous matrix, including proteoglycans and type II collagen, also showed prominent increasing in the TETA group. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining showed that the apoptosis of seeded chondrocytes was attenuated in the presence of TETA.

 

CONCLUSION: We demonstrated that TETA protected the chondrocyte-seeded construct from deformation after in vivo cultivation, increased the chondrocytes density and cartilaginous matrix and decreased apoptotic cell death in the construct. Our work provides a novel approach to replace muscle flap and simultaneously accelerate chondrogenesis which can be applied in tracheal tissue engineering for the treatment of tracheal resection patients.

 

  1. Tsao C-K, Ko C-Y, Yang S-R, et al. An ectopic approach for engineering a vascularized tracheal substitute. Biomaterials. 2014;35(4):1163-1175.