Abstract: A New Regenerative Therapy of Elastic Cartilage Grafting for Craniofacial Deformities (Plastic Surgery The Meeting)

Saturday, September 24, 2016

Shintaro Kagimoto, MD, PhD , Plastic Surgery, Yokohama City University, Yokohama, Japan

Yuichiro Yabuki, MD , Plastic Surgery, Department of Plastic and Reconstructive Surgery, Kanagawa Children's Medical Center, Yokohama, Japan

Koichi Hirotomi, MD , Plastic Surgery, Yokohama City University medical center, Yokohama, Japan

Taro Mikami, MD，PhD , Plastic Surgery, Yokohama City University Hospital, Yokohama, Japan

Takanori Takebe, MD, Professor , Regenerative Medicine, Yokohama city university, Yokohama city, Japan

Shinji Kobayashi, MD, PhD , Plastic Surgery, Department of Plastic and Reconstructive Surgery, Kanagawa Children's Medical Center, Yokohama, Japan

Hideki Taniguchi, MD, PhD, Professor , Regenerative Medicine, Yokohama city university, Yokohama, Japan

Jiro Maegawa, MD, PhD, Professor , Plastic Surgery, Yokohama City University, Yokohama, Japan

Presentation PDF file

E-Poster

Background

Craniofacial injuries and abnormalities such as deviated nose and microtia etc., are often treated by self-cartilage grafting. For autologous tissue grafting, limitations include the collectable volume, donor site morbidity, and tissue absorption; more importantly, the long-term clinical outcomes remain quite poor due to tissue maintenance failures

We recently identified a promising regenerative method based on the xenotransplantation of human cartilage progenitor cells in immunodeficient animals(1) and autotransplantation of monkey cartilage progenitors cells (2) to reconstruct self-renewing elastic cartilage. The aim of this study was to control the volume and shape of self-renewing elastic cartilage by two another methods as a pre-clinical study.

Methods

Three monkeys (Macaca fascicularis) were used. First, we identified the progenitor population from the monkey ear perichondrium. Progenitor cells were expanded and differentiated extensively into chondrocytes in vitro. First group(Two-stage grafting(3)) was atuotransplanted the differentiated progenitor cells into monkeys abdominal lesions, after 6 month collect reconstructive tissue ,process them and re-autotransplanted craniofacial area. Second group(Using rotating wall vessel bioreactor(4)) was added cutured the differentiated progenitor cells using rotating wall vessel bioreactor(RWV) in vitro 3 weeks, then autotransplanted craniofacial area. This autotransplantated tissue regeneration was monitored using non-invasive magnetic resonance imaging (MRI). The transplanted cells were retrieved three months later. Histological assessment and glycosaminoglycan (GAG) levels of reconstructed tissue were assessed.

The experimental animal protocols were approved by the Animal Welfare and Animal Care Committee of the National Institute of Biomedical Innovation (Osaka, Japan)(approval ID: DS25-32).

Results

Remarkable formation change was detected in both of 2 groups clinically. We also detected density difference in the MRI T1/T2 images. Elastic cartilage was detected in histological assessment, GAG levels were nearby original auricular cartilage. No complication such as inflammation and tumorigenesis were observed in any of the experiments.

Conclusion

These 2 methods will be a highly promising to treat craniofacial deformities. We conclude that to use properly these methods will be needed for patient condition.

30054 A New Regenerative Therapy of Elastic Cartilage Grafting for Craniofacial Deformities